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The genome size of p_ctg.fa is smaller than survey #669

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Pengzw0909 opened this issue Jun 25, 2024 · 1 comment
Open

The genome size of p_ctg.fa is smaller than survey #669

Pengzw0909 opened this issue Jun 25, 2024 · 1 comment

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@Pengzw0909
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Hi,
I did a NGS survey and a TGS survey, the estimated size is about 2.2Gb.The genome size of p_ctg.fa is 1.69Gb
, smaller than survey. Can you give me some suggestion? It‘s an insect of diptera.

NGS survey:
image

TGS survey:
image

cmd:
fa=ccs.fasta
~/01.software/hifiasm/hifiasm-0.19.8/hifiasm -o hifiasm.asm -t60 -l 2 -n 4 $fa 2> $prefix.assemble.log

p_ctg.fa:
Contigs stat:
CtgNum CtgLen CtgN50 CtgN90 CtgMax GC(%)
1029 1693753142 18588588 2976463 107319031 39.83

the log is
assemble.log

So I have changed the parameter “--hom-cov=57”,the genome size is 2.8Gb, but I found that there was a lot of redundancy. After purge,the genome size is smaller than 2.2Gb.

@chhylp123
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2.2 Gb assembly with purged might be right?

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