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Hi,
I did a NGS survey and a TGS survey, the estimated size is about 2.2Gb.The genome size of p_ctg.fa is 1.69Gb
, smaller than survey. Can you give me some suggestion? It‘s an insect of diptera.
So I have changed the parameter “--hom-cov=57”,the genome size is 2.8Gb, but I found that there was a lot of redundancy. After purge,the genome size is smaller than 2.2Gb.
The text was updated successfully, but these errors were encountered:
Hi,
I did a NGS survey and a TGS survey, the estimated size is about 2.2Gb.The genome size of p_ctg.fa is 1.69Gb
, smaller than survey. Can you give me some suggestion? It‘s an insect of diptera.
NGS survey:
![image](https://private-user-images.githubusercontent.com/34698068/342630965-5768cc1e-9fb7-46c0-be9d-88968a66fe60.png?jwt=eyJhbGciOiJIUzI1NiIsInR5cCI6IkpXVCJ9.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.7j9GidaZ2vx6FUh6Iaukg3UbHDA0Wh7zC7slQk8zeQU)
TGS survey:
![image](https://private-user-images.githubusercontent.com/34698068/342631122-f90f42c8-bcea-423d-b2fd-c61ec81c1c05.png?jwt=eyJhbGciOiJIUzI1NiIsInR5cCI6IkpXVCJ9.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.F8UXRy0qQUqCnPBJlLYV7-Wg_TXb6rG4DHwjsocM9dQ)
cmd:
fa=ccs.fasta
~/01.software/hifiasm/hifiasm-0.19.8/hifiasm -o hifiasm.asm -t60 -l 2 -n 4 $fa 2> $prefix.assemble.log
p_ctg.fa:
Contigs stat:
CtgNum CtgLen CtgN50 CtgN90 CtgMax GC(%)
1029 1693753142 18588588 2976463 107319031 39.83
the log is
assemble.log
So I have changed the parameter “--hom-cov=57”,the genome size is 2.8Gb, but I found that there was a lot of redundancy. After purge,the genome size is smaller than 2.2Gb.
The text was updated successfully, but these errors were encountered: