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Extracting methylation haplotypes from sequence alignment files #10

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meb326 opened this issue Sep 15, 2019 · 3 comments
Open

Extracting methylation haplotypes from sequence alignment files #10

meb326 opened this issue Sep 15, 2019 · 3 comments

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@meb326
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meb326 commented Sep 15, 2019

Hi dinhdiep,

I'm very interested in your work and want to conduct methylation haplotype analysis on my bisulfite sequencing data. I am having trouble generating CpG haplotype files using your code. When I try the test example:
./scripts/bam2cghap.sh allcpg/cpg.small.txt.gz BAMfiles/Colon_primary_tumor_sept9_promoter.bam test

I get this error for many lines:
Use of uninitialized value $ref_seq in concatenation (.) or string at bin/getHaplo_PE_cgOnly.pl line 161, line 28.

Could you help me to get this to work? I would really appreciate it.

@long692108

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@long692108
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I am also interested in this work. Did you have solve this problem?

@kythol
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kythol commented May 9, 2023

I have the same error

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