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add-host is failing on mask_host #98
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Hello, I have also encountered the same error and fail to solve this problem. It would be very helpful to let me know if you have any sollutions, thanks a lot! |
[2023:11:16 13:41:28] Config file hecatomb.out/hecatomb.config.yaml already exists. Using existing config file. args: [2023:11:16 13:41:28] --------------------- snakemake -s /public3/home/sc/anaconda3/envs/hecatomb/lib/python3.10/site-packages/hecatomb/snakemake/workflow/AddHost.smk --configfile hecatomb.out/hecatomb.config.yaml --jobs 8 --use-conda --conda-prefix /public3/home/sc/anaconda3/envs/hecatomb/lib/python3.10/site-packages/hecatomb/snakemake/conda --rerun-incomplete --printshellcmds --nolock --show-failed-logs all 1 Select jobs to execute... [Thu Nov 16 13:41:32 2023]
Activating conda environment: ../../anaconda3/envs/hecatomb/lib/python3.10/site-packages/hecatomb/snakemake/conda/2529cd9812bb3d1ebf33334bfdd3a3f8_
Logfile hecatomb.out/stderr/mask_host.log:java -ea -Xmx32000m -Xms32000m -cp /public3/home/sc/anaconda3/envs/hecatomb/lib/python3.10/site-packages/hecatomb/snakemake/conda/2529cd9812bb3d1ebf33334bfdd3a3f8_/opt/bbmap-38.90-3/current/ jgi.BBMask in=db/gga.fa out=hecatomb.out/processing/temp/gga.processed.fasta entropy= sam=hecatomb.out/processing/temp/gga.sam.gz ow=t threads=8 -Xmx32000m Exception in thread "main" java.lang.NullPointerException
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I've reworked these rules and it seems to be working. I've dropped BBTools for minimap and BEDtools, and just using the normal refseq viral db rather than the shredded version. You can test it like so: git clone https://github.com/shandley/hecatomb.git
cd hecatomb
git checkout dev
conda create -n hecatombDev python=3.11
conda activate hecatombDev
pip install -e .
cd ..
hecatomb add-host --host yeet --host-fa GCA_000001405.29_GRCh38.p14_genomic.fna --threads 8
hecatomb list-hosts |
I managed to replicate the issue:
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