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adamjtaylor committed Feb 10, 2024
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Expand Up @@ -61,9 +61,11 @@ Other columns may be provided but are not used by the pipeline.
- `input`: Path to a CSV sample sheet. This parameter is required.
- `outDir`: Specifies the directory where the output data should be saved. Default is `outputs`.

#### HistoQC options
#### Other options

- `config`: Configuration file used by HistoQC. Must be one of `default`, `ihc`, `clinical`, `first`, `light`, or `v2.1`.Custom config files are not currently supported.
- `config` (string): Name of a built-in configuration used by HistoQC. Must be one of `default`, `ihc`, `clinical`, `first`, `light`, or `v2.1`. Defaults to `default`.
- `custom_config` (path): Path to a HistoQC compatible configuration file. Must have a `.ini` extension. Overrides `config`.
- `convert` (bool): If provided, `vips` is used to [create an OpenSlide compatiable TIFF file](http://www.andrewjanowczyk.com/converting-an-existing-image-into-an-openslide-compatible-format/). Uses [mc2-center/histoqc-openslide-converter](https://github.com/mc2-center/histoqc-openslide-converter).

### Profiles

Expand All @@ -86,23 +88,45 @@ The container is automatically pulled by NextFlow, but if local use is required
> A Nextflow pipeline is implicitly modelled by a direct acyclic graph (DAG). The vertices in the graph represent the pipeline’s processes and operators, while the edges represent the data connections (i.e. channels) between them.
```mermaid
flowchart TD
p0((Channel.fromPath))
p1([splitCsv])
p2([map])
p3[NF_HISTOQC:RUN_HISTOQC:HISTOQC]
p4(( ))
p5(( ))
p6([collect])
p7[NF_HISTOQC:COLLECT_RESULTS:COLLECT]
p8(( ))
p0 --> p1
p1 --> p2
p2 -->|images| p3
p3 -->|results| p6
p3 --> p5
p3 -->|output| p4
p6 --> p7
p7 --> p8
flowchart TB
subgraph " "
v0["Channel.fromPath"]
v3["Channel.fromPath"]
v6["config_string"]
end
subgraph NF_HISTOQC
subgraph RUN
v5([CONVERT])
v7([HISTOQC])
v1(( ))
v4(( ))
v9(( ))
v13(( ))
end
subgraph COLLECT
v10([RESULTS])
v11([TIDY])
v14([LOGS])
end
end
subgraph " "
v8["output"]
v12[" "]
v15[" "]
end
v0 --> v1
v3 --> v4
v1 --> v5
v5 --> v7
v6 --> v7
v4 --> v7
v7 --> v8
v7 --> v9
v7 --> v13
v9 --> v10
v10 --> v11
v11 --> v12
v13 --> v14
v14 --> v15
```

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