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slideQuantify v2: 2 ‐ Extract Tiles

timpeters03 edited this page Feb 15, 2024 · 3 revisions

Description:
This script facilitates the extraction of tiles from images, the second process within the slideQuantify workflow. It operates in a SLURM-based environment and utilizes slideExtractTiles.py within slideToolKit for tile extraction.

  • Written by: Tim S. Peters
  • E-mail: [email protected]
  • Last update: 2024-01-09
  • Version: 1.0.0

Usage:

./slideQuantify_v2_2_extract_tiles.sh [project_dir] [masks_dir] [layer] [tile_size] [study_number]

Example Usage:

./slideQuantify_v2_2_extract_tiles.sh /path/to/project_dir /path/to/masks_dir 1 2000 AE21

Arguments:

  • project_dir
    • Description: Path to the project directory containing _ndpi and/or _tif folders with images.
    • Required: Yes
  • masks_dir
    • Description: Path to the directory where masks for the images will be stored.
    • Required: Yes
  • layer
    • Description: Magnification layer of the image from which tiles should be extracted.
    • Required: Yes
  • tile_size
    • Description: Size of the extracted tiles.
    • Required: Yes
  • study_number
    • Description: Study number of the processed image.
    • Required: Yes

Overview

Welcome to slideToolKit

Manual

Introduction
General instructions

slideToolKit scripts

slide2Tiles
slideAppend.sh
slideAppendGCT.sh
slideConvert
slideDirectory
slideDupIdentify.py
slideEMask
slideEntropySegmentation.py
slideExtract.py
slideExtractTiles.py
slideInfo
slideInfo.py
slideJobChecker
slideLookup
slideMacro
slideMacro.py
slideMask
slideNormalize
slideRename
slideRename.py
slideThumb
slideThumb.py

slideQuantify v1

slideQuantify_v1
slideQuantify_v1_1_expresshist_mask.sh
slideQuantify_v1_2_expresshist_tile.sh
slideQuantify_v1_3_tile_normalizing.sh
slideQuantify_v1_4_cellprofiler.sh
slideQuantify_v1_5_wrapup.sh

slideQuantify v2

slideQuantify_v2
slideQuantify_v2_1_entropy_segmentation.sh
slideQuantify_v2_2_extract_tiles.sh
slideQuantify_v2_3_tile_normalizing.sh
slideQuantify_v2_4_cellprofiler.sh
slideQuantify_v2_5_wrapup.sh

slideQuantifyOSX

slideQuantifyOSX
slideQuantify_cellprofiler.sh
slideQuantify_mask.sh
slideQuantify_normalizing.sh
slideQuantify_tiling.sh
slideQuantify_wrapup.sh

Other scripts

slideToolKitTest.py

Installation

macOSX

Conda version (default/preferred)
Homebrew version

Linux

Rocky 8 Conda version (default/preferred)

Legacy

Ubuntu 16.04 LTS
Ubuntu 12.04 CentOS7 Conda version with modules
Administrator version

Conda vs brew

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